Coding
Part:BBa_K313002:Design
Designed by: Kohaku So, Takashi Hiroi, Megumi Iseki, Mami Nakatake, Ryosuke Kamei, Ryo Kariyazono Group: iGEM10_UT-Tokyo (2010-10-14)
flpe DNA recombinase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 504
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
It's internal EcoRI and SpeI site has been removed using site-directed mutagenesis.
Source
E.coli EL250 strain